The effect of atmospheric cold plasma treatment on the antigenic properties of bovine milk casein and whey proteins

Casein, ?-lactoglobulin and ?-lactalbumin are major milk protein allergens. In the present study, structural modifications antigenic response of these bovine allergens as induced by non-thermal treatment atmospheric cold plasma were investigated. Spark discharge (SD) glow (GD), previously characterized systems, used for treatments. analyzed before after using SDS-PAGE, FTIR, UPLC-MS/MS ELISA. SDS-PAGE results revealed a reduction in casein intensity bands SD or GD treatments; however, band remained unchanged. FTIR studies alterations secondary structure plasma, particularly contents ?-sheet ?-turn. The showed that amino acid compositions decreased ELISA decrease antigenicity post treatment, whereas an increase antigenicity. study indicates can be tailored to mitigate risk dairy processing ingredients sectors.